Aspirin prevents metastasis by limiting platelet TXA2 suppression of immunity

Mice and reagents All animal experiments were conducted in accordance with UK Home Office guidelines and were approved by the University of Cambridge Animal Welfare and Ethics Review Board. Littermate controls or age- and sex-matched mice were used in experiments as indicated. All mice, unless otherwise stated, were housed at the University of Cambridge University Biomedical Services Gurdon Facility or the Babraham Institute Biological Support Unit. Wild-type C57BL/6 mice were obtained from Charles River. Ptprca (CD45.1) congenic, OT-1 TCRtg, Rag2–/– and MMTV-PyMT (B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ) mice were obtained from the Jackson Laboratory. Arhgef1-KO (Arhgef1Tm1a) mice have previously been described12. Tbxas1-KO (Tbxas1tm1Swl) and Tbxa2R-KO (Tbxa2rtm1Cof) mice, as previously described44,49, were provided by S.-W. Lin. Arhgef1fl/fl mice were generated by crossing Arhgef1Tm1a mice with FlpO-deleter mice50, and subsequently crossed with Cre-expressing strains, Ncr1cre (Ncr1tm1.1(ic ... Read full article.