Adipose tissue retains an epigenetic memory of obesity after weight loss

Data reporting No statistical methods were used to predetermine sample size. The experiments were not randomized, and the investigators were not blinded to allocation during experiments and outcome assessment. Clinical sample acquisition Human AT biopsies were obtained from three independent studies: MTSS, LTSS and NEFA. MTSS The MTSS samples comprised samples from omental visceral AT biopsies obtained in the context of a two-step BaS treatment, which included a sleeve gastrectomy as the first step (T0) and laparoscopic RYGB as the second step (T1)16. Individuals with syndromal, monogenic, early-onset obesity or individuals with other known concurrent diseases, including acute infections or malignant diseases, were not included in the study. Individuals were not required to adhere to any specific diet before or after surgery but received individual dietary recommendations during regular visits in the obesity management centre. Insulin resistance was determined using a hyperinsulin ... Read full article.