GoKawiilAnimals
Animal experiments were performed following standard ethical guidelines (European Communities Guidelines on the Care and Use of Laboratory Animals, 86/609/EEC), and were approved by the Veterinary Department of the Canton of Basel-Stadt. C57BL6, PV-cre (The Jackson Laboratory, 008069) mice and PV-cre mice crossed with Ai9(RCL-tdT) mice (The Jackson Laboratory, 007909) in the C57BL6 background were used at 55–120 days old. Both females and males were used for this study. They were maintained on a normal 12 h–12 h light–dark cycle in a pathogen-free environment with ad libitum access to food and drinking water. Mice were euthanized by exposure to CO 2 . No formal statistical methods were used to predetermine sample sizes. Mice were allocated randomly to treatment conditions. No blinding to group allocation was performed during the experiments.
Post-mortem human retina donations
Human retina tissue was obtained from multi-organ donors through the sampling of non-transplantable eye tissue extracted during cornea collection for transplantation. Retinas from individuals with a documented history of eye disease were excluded. The collection of tissue samples adhered to the principles outlined in the Declaration of Helsinki, and all experimental protocols received the approval of the local ethics committee.
HEK293T cells
All cell cultures were maintained at 37 °C in a humidified incubator (5% CO 2 ). HEK293T cells (ATCC, CRL-3216) were cultured in DMEM (Gibco, 10566016) supplemented with 10% FBS and 1% penicillin–streptomycin (Gibco, 15140-122). Cells were passaged at 90% confluency. Cell dissociation was performed using TrypLE Express (Thermo Fisher Scientific, 12604021).
Bone marrow mMSCs
Bone marrow mMSCs were obtained from Cyagen (MUBMX-01001). Cells were cultured in MesenCult Proliferation medium (StemCell Technologies, 05411) up to passage 11. Cells were passaged with TrypLE Express at 70–80% confluency.
Human cardiac cells
Human cardiac cells isolated from the ventricles of the adult heart (Promocell, C-12810) were maintained in myocyte growth medium (Promocell, C-22070). For passaging, cells were detached with TrypLE Express at 70% confluency and seeded at a density of 10,000–15,000 cells per cm2.
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