In the originally published version of this article, an error in one version of the code used for segmentation of individual human presomitic mesoderm (PSM) cells from three time-lapse microscopy datasets caused properly detected cells to be duplicated and incorrectly included extra objects that did not meet the minimum size criteria. The result was a complete or partial duplication of single-cell tracks and Hes7-Achilles oscillation profiles for these three datasets. All other datasets in this article were correctly processed with an alternative version of the code and were unaffected by duplications. The impacted datasets have been re-analyzed using the correct version of the batch-processing segmentation code and all downstream analyses have been redone. Figures 1f, 3a, 3d, 4b, 4d, 4e and 4f and Extended Data Figs. 5f, 6h, 6i and 6n, as well as the source data, have been updated accordingly in the Supplementary Information accompanying this amendment (due to the age of the article, the figures cannot be updated directly). Because all cells to be detected were added twice and only a few (if any) additional regions were included, the analyses and statistics were only marginally affected, and all statements remain accurate. All claims made in the original version of this article remain well supported by the corrected data, with similar statistical power.
Author Correction: In vitro characterization of the human segmentation clock
Why This Matters
This correction highlights the importance of rigorous data validation in scientific research, especially in complex image analysis workflows. Ensuring accuracy in such processes is crucial for maintaining trust and reliability in published findings, which can influence future studies and technological advancements in developmental biology. The update reassures the scientific community that the core conclusions remain valid despite the initial coding error.
Key Takeaways
- Accurate segmentation code is vital for reliable cell tracking in microscopy datasets.
- Even minor coding errors can impact data integrity, underscoring the need for thorough validation.
- The corrected analysis confirms that key findings of the study remain robust and trustworthy.
Explore topics:
human segmentation clock
presomitic mesoderm
hes7-achilles
time-lapse microscopy
batch-processing segmentation
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