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Structural basis of the RNA-editing cascade in trypanosome mitochondria

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Why This Matters

This research uncovers the detailed structural mechanisms behind RNA editing in trypanosome mitochondria, a process essential for parasite survival and pathogenicity. Understanding these molecular pathways offers potential avenues for targeted treatments against diseases like sleeping sickness. For the tech industry, these insights could inspire innovative RNA-based technologies and enhance our understanding of mitochondrial gene regulation.

Key Takeaways

Benne, R. et al. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA. Cell 46, 819–826 (1986).

Blum, B., Bakalara, N. & Simpson, L. A model for RNA editing in kinetoplastid mitochondria: RNA molecules transcribed from maxicircle DNA provide the edited information. Cell 60, 189–198 (1990).

Seiwert, S. D., Heidmann, S. & Stuart, K. Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing. Cell 84, 831–841 (1996).

Kable, M. L., Seiwert, S. D., Heidmann, S. & Stuart, K. RNA editing: a mechanism for gRNA-specified uridylate insertion into precursor mRNA. Science 273, 1189–1195 (1996).

Aphasizheva, I. & Aphasizhev, R. U-insertion/deletion mRNA-editing holoenzyme: definition in sight. Trends Parasitol. 13, 1078–1083 (2015).

Aphasizheva, I. et al. Lexis and grammar of mitochondrial RNA processing in trypanosomes. Trends Parasitol. 36, 337–355 (2020).

Blum, B. & Simpson, L. Guide RNAs in kinetoplastid mitochondria have a nonencoded 3’ oligo-(U) tail involved in recognition of the pre-edited region. Cell 62, 391–397 (1990).

Blum, B. & Simpson, L. Formation of gRNA/mRNA chimeric molecules in vitro, the initial step of RNA editing, is dependent on an anchor sequence. Proc. Natl Acad. Sci. USA 89, 11944–11948 (1992).

Liu, S. et al. Structural basis of gRNA stabilization and mRNA recognition in trypanosomal RNA editing. Science 381, eadg4725 (2023).

Trotter, J. R., Ernst, N. L., Carnes, J., Panicucci, B. & Stuart, K. A deletion site editing endonuclease in Trypanosoma brucei. Mol. Cell 20, 403–412 (2005).

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