Plants monitor the integrity of their barrier by sensing gas diffusion
Plant materials and growth conditions Col-0 was used as the wild type unless stated otherwise. ein2-1, etr1-3, ate1-2;ate2-1, prt6-5, proWOX4-erYFP, proPXY:GUS, proPXY:erVenus and 35S:EIN3-GFP have been described previously10,13,27,37,38,39,40. ate1-2;ate2-1 mutants were reciprocally backcrossed into Col-0 for three generations and transfer DNA insertions were confirmed by genotyping to obtain double-homozygous mutants. Seeds were sown on half-strength MS (Duchefa) plates supplemented with 0.0