GoKawiila, Diagram illustrating the construction of the BC 2 F 6 population used for this study by crossing Mo17 to CIMBL55. b, Correlation analysis of days to anthesis (DTA), days to silking (DTS), and anthesis–silking interval (ASI) for plants under flowering drought (FD) conditions. The histograms along the diagonal show the phenotypic distribution of each trait. The values in the upper-right triangle indicate the pairwise correlation coefficients between two traits, and the plots in the lower-left triangle are the corresponding scatterplots of the two traits. Red asterisks indicate a significant correlation at **, P < 0.01, ***, P < 0.001. c, Statistic summary of the flowering traits of the BC 2 F 6 population. d, Diagrams illustrating the measurement of the lengths of the tassels, ears as well as silks. Scale bars are shown. e, qDR9 was originally mapped between markers M1 and SVM7-1 on chromosome 9 using 315 BC 2 F 6 lines. qDR9 was narrowed down to a 614-kb genomic region flanked by markers M4-2 and KASP-11 using 5,227 individuals from a BC 2 F 7 population generated from the BC 2 F 6 lines that were heterozygous at qDR9 but not at other QTL regions in the 2021 field trial. f, The effective region underlying qDR9 was further delimited to a 5,299-bp genomic region flanked by markers C0 and KASP6 using 2,897 individuals from a BC 2 F 8 population generated from the BC 2 F 7 lines that were heterozygous over the 614-kb region in the 2022 field trial. The number of recombinants between adjacent markers is indicated below the linkage map. The genotypes of the critical recombinants are shown below the chromosome segments showing the positions of markers. Mint green, purple, and gray segments indicate regions homozygous for CIMBL55, Mo17 and heterozygous regions, respectively. The bar graphs at right report the ASI values between homozygous recombinants and homozygous nonrecombinants within each recombinant-derived F 3 family. Comparisons between the two F 3 families derived from recombinants R10, R11, and R15 are also presented in Fig. 1a. The sample size (n, plant number) for each group is indicated on the left of each column. Data are means ±s.d.; P values were determined by two-tailed Student’s t-tests.
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