GoKawiilStudy details biochemical method revealing how spliceosomes disassemble after splicing
Researchers used nematode (C. elegans) extract containing a FLAG-tagged PRP19 protein to study how the intron lariat spliceosome (ILS) complex breaks apart once its job is done. By comparing protein pulldowns with and without ATP, they identified which factors are released from the spliceosome specifically when ATP is present, pointing to an ATP-dependent disassembly step. Mass spectrometry and statistical analysis were used to pinpoint proteins significantly depleted under ATP treatment.