GoKawiilAllen Institute maps 3D structure of noradrenaline neurons in mouse brain's locus coeruleus
Researchers at the Allen Institute and Johns Hopkins used Dbh-Cre mice with fluorescently labeled nuclei, tissue clearing, and light-sheet microscopy to image and count noradrenaline-producing neurons in the locus coeruleus at cellular resolution. They applied a deep-learning pipeline to detect neuron centroids and registered the data to a standard brain atlas to build detailed 3D density maps of this small but influential brainstem nucleus.