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Polymerase

2 GoKawiil briefs on this topic

Thermo Fisher Scientific Produces Monkeypox Virus Replisome Proteins for Study

Researchers expressed and purified key proteins from the monkeypox virus using Expi293F cells, including the viral polymerase complex components. The proteins were cloned, transfected, and isolated through standard cell lysis and affinity purification methods. This work enables detailed analysis of the virus's replication machinery.

New automated platform speeds design and testing of synthetic genetic codes

Researchers describe a workflow that synthesizes and in-vitro-tests large pools of tRNA variants, including E. coli, M. alvus and M. mazei isoacceptors, using microchip-derived DNA oligo pools, T7-driven transcription, and cell-free translation systems. The method allows rapid amplification, refolding, purification and aminoacylation testing of tRNAs to evaluate their function outside living cells.