GoKawiilNew 'Prime Assembly' method enables large-scale gene insertion using prime editing
Researchers used K562, Jurkat and HEK293T cell lines to test a genome-editing technique called prime assembly, which combines prime editing components (PE7 or PEmax) with pegRNAs and various DNA donor templates to insert genetic sequences at targeted genomic sites. The team tested donor formats ranging from short synthetic single-stranded DNA to large double-stranded DNA fragments up to 12.1 kilobases, optimizing nucleofection conditions for each donor type.