GoKawiilStudy details TRAM's role in Myddosome signaling independent of Toll-like receptors
Researchers describe laboratory methods used to study TRAM (Ticam2), a signaling adaptor, showing it can promote myddosome formation without direct involvement of Toll-like receptors. The work used immortalized and primary bone-marrow-derived macrophages from wild-type and Ticam2-knockout mice, alongside HEK293T cells, treated with bacterial components such as LPS, LTA and Pam2CSK4 to probe the pathway.