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Alpacas produce VHH antibodies targeting human IL-10 receptors via immunization and phage display

Six alpacas were immunized with components of the human IL-10 receptor, and their blood samples were used to create a VHH phage library. This library was subjected to multiple rounds of panning against IL-10 receptor subunits to isolate specific antibody fragments. Additionally, humanized and knockout mouse lines were generated for further research applications.