a, Mean brain concentration-time profile of DOI-NBOMe following intraperitoneal administration (8 mg/kg; n = 3). b, c, Antagonism of DOI (1 mg/kg, i.p.)- (b) or LSD (0.24 mg/kg, i.p.)- (c) induced HTR by DOI-NBOMe pretreatment (2 mg/kg, i.p.). The number of mice for each group is indicated direct below the data for each group. Data are expressed as mean ± s.e.m.; **p < 0.002, ***p < 0.001; (two-tailed unpaired t-test). d, e, Schematic of the corticosterone-induced depression like model and schematic of the SPT (d), OFT and FST (e). f, Long-term effects of DOI-NBOMe in forced swim test after 7 days of drug administration. The number of mice for each group is indicated direct below the data for each group. Data are expressed as mean ± s.e.m.; *p < 0.033, ns, not significant; (two-way repeated-measures ANOVA with Dunnett’s test). g, h, Effects of DOI-NBOMe on OFT in a corticosterone-induced depression-like mice model. The number of mice for each group is indicated direct below the data for each group. Data are expressed as mean ± s.e.m.; ***p < 0.001; ns, not significant; (two-way repeated-measures ANOVA with Dunnett’s test). i, Schematic of drug administration and marble-burying test (MBT). j, Total number of marbles buried during the MBT for mice after 24 h injected with DOI, DOI-NBOMe and ariadne. The number of mice for each group is indicated direct below the data for each group. Data are expressed as mean ± s.e.m.; *p < 0.033; **p < 0.002; ns, not significant. (one-way repeated-measures ANOVA with Dunnett’s test). Illustrations in d, e and i were created in BioRender. Xia, F. (2025) https://biorender.com/ri5f9iu.
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