GoKawiila. Counts for all macrophages and CD45.1 WT macrophages in the aorta of Ldlr−/−Jak2V617FBM and Ldlr−/−WTBM mice measured by flow cytometry at the time of euthanasia (n = 5, 5 wt; 6, 6 sed; 10, 11 SF and 6, 6 exercise). b. CD45.2+ aortic macrophage proliferation measured by flow cytometry in Ldlr−/−Jak2V617FBM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 5 wt, 7 sed, 11 SF, 6 exercise). c. Representative flow cytometry plots for bead uptake into aortic macrophages in Ldlr−/−Jak2V617FBM and Ldlr−/−WTBM control mice with quantification of percent of bead-positive macrophages across all lifestyle groups (n = 5, 6 wt; 5, 5 sed; 6, 6 SF and 7, 7 exercise for CD45.1 and CD45.2, respectively). d. Correlation between the fractions of CD45.2 monocytes in the blood and CD45.2 macrophages in the aorta of Ldlr−/−Jak2V617FBM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 41, 41 for blood and aorta, respectively). e. Counts for all macrophages and CD45.1 WT macrophages in the aorta of Ldlr−/−Tet2−/−BM and Ldlr−/−WTBM mice measured by flow cytometry at the time of euthanasia (n = 6, 6 wt; 7, 7 sed; 6, 7 SF and 6, 6 exercise). f. CD45.2+ aortic macrophage proliferation measured by flow cytometry in Ldlr−/−Tet2−/−BM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 6 wt, 7 sed, 7 SF and 6 exercise). g. Quantification of bead uptake into aortic macrophages in Ldlr−/−Tet2−/−BM mice across all lifestyle groups (4, 4 sed; 5, 5 SF and 4, 4 exercise). h. Correlation between the fractions of CD45.2 monocytes in the blood and CD45.2 macrophages in the aorta of Ldlr−/−Tet2−/−BM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 26, 26 for blood and aorta, respectively). i. Counts for all macrophages and CD45.1 WT macrophages in the aorta of Ldlr−/−p53−/−BM and Ldlr−/−WTBM mice measured by flow cytometry at the time of euthanasia (n = 4, 4 wt; 8, 8 sed; 9, 8 SF and 8, 8 exercise). j. Quantification of bead uptake into aortic macrophages in Ldlr−/− p53−/−BM mice across all lifestyle groups (4, 4 sed; 5, 5 SF and 4, 4 exercise for CD45.1 and CD45.2, respectively). k. Correlation between the fractions of CD45.2 monocytes in the blood and CD45.2 macrophages in the aorta of Ldlr−/−p53−/−BM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 29, 29 for blood and aorta, respectively). l. Lesion size, volume and necrotic core area (n = 5 wt, 6 sed, 6 SF, 7 exercise) with representative images of aortic roots stained with Masson’s Trichrome stain, quantification of total and CD45.2 aortic macrophage number, and CD45.2 macrophage proliferation in Ldlr−/−Dnmt3aR878HBM and Ldlr−/−WTBM control mice at the time of euthanasia (n = 4 wt, 7 sed, 7 SF, 4 exercise); scale bar=200 µm. Two-sided statistics. Data are mean ± s.e.m; *p < 0.05, **p < 0.01. P-values were obtained from independent, two-tailed, Welch’s t-tests (for parametric data) or Mann–Whitney U tests (for non-parametric data). Schematics in panels a,c,e,i–l created in BioRender; McAlpine, C. https://biorender.com/1rt7rau (2026).
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