GoKawiilStudy models mouse embryo development using chemically induced totipotent stem cells
Researchers generated chemically induced totipotent-like (ciToti) stem cell structures and tracked their single-cell transcriptomes across multiple stages, comparing them to natural mouse blastocysts at E3.5 and E4.5. The analysis identified progenitor and intermediate cell populations transitioning toward epiblast, trophectoderm and primitive endoderm lineages, mapping their developmental trajectories using pseudotime and distance-based methods.
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The findings suggest that chemically induced totipotent cells can recapitulate key lineage specification events seen in natural embryo development, which could make them a useful model for studying early mammalian development without relying on embryos. This approach may help researchers probe questions about cell fate decisions and totipotency that are otherwise difficult to study in vivo.
- Chemically induced totipotent (ciToti) stem cell structures were compared to natural mouse blastocysts using single-cell RNA sequencing.
- Intermediate cell populations were identified that show transitional characteristics between trophectoderm, epiblast and primitive endoderm identities.
- Pseudotime and distance-based analyses were used to map developmental trajectories of these stem cell-derived structures.
Source: nature.com — Wu, 2026-10-14
Published there as: “Modelling mouse embryogenesis from chemically induced totipotent stem cells”
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