GoKawiilStudy models mouse embryo development using chemically induced totipotent stem cells
Researchers generated chemically induced totipotent-like (ciToti) stem cell structures and tracked their single-cell transcriptomes across multiple stages, comparing them to natural mouse blastocysts at E3.5 and E4.5. The analysis identified progenitor and intermediate cell populations transitioning toward epiblast, trophectoderm and primitive endoderm lineages, mapping their developmental trajectories using pseudotime and distance-based methods.