Scrna Seq - GoKawiil

Study links serpin proteins to pancreatic cancer subtype diversity and immune escape

Researchers combined single-cell RNA sequencing from human and mouse pancreatic tumours with protein localization databases, CRISPR dependency screens and functional annotation to identify tumour-secreted genes that shape cancer cell diversity and suppress immune responses. The filtering pipeline pinpointed a group of serpin family proteins acting through myeloid cells as key drivers of this immune evasion process. The work narrows a large pool of candidate genes down to those most likely to influence how pancreatic tumours interact with surrounding immune cells.

nature.com · 2026-09-09

CIGMA tool maps how gene expression variants differ across individual cell types

Researchers introduced CIGMA, a statistical method that uses a linear mixed model to separate genetic effects on gene expression into components shared across cell types and components specific to individual cell types, applied to single-cell RNA sequencing data. The model uses cell-type-level pseudobulk expression averaged from single-cell data, requiring more than 10 cells per individual per cell type, and explicitly accounts for cell-to-cell noise within each individual and cell type.

nature.com · 2026-08-26