GoKawiilNew automated platform speeds design and testing of synthetic genetic codes
Researchers describe a workflow that synthesizes and in-vitro-tests large pools of tRNA variants, including E. coli, M. alvus and M. mazei isoacceptors, using microchip-derived DNA oligo pools, T7-driven transcription, and cell-free translation systems. The method allows rapid amplification, refolding, purification and aminoacylation testing of tRNAs to evaluate their function outside living cells.