GoKawiilEngineered E. coli Ribo-T ribosomes shown to translate proteins from their own rRNA
Researchers modified the tethered Ribo-T ribosome system in E. coli by appending open reading frames for GFP, DFR and HiBiT directly onto the 3′ end of 16S rRNA, creating 'Ribo-TM' constructs. Gel electrophoresis and RT-PCR confirmed the fused mRNA-rRNA molecules were intact and correctly assembled inside functioning ribosomes, and growth assays with mutant ribosomal subunits helped verify that translation activity depended on a functional ribosome.