Published on: 2025-05-27 16:33:11
CRISPRa browning of human white adipocytes To induce browning in human adipocytes, we used CRISPRa to upregulate UCP1, PPARGC1A or PRDM16, all known genes involved in BAT development and function. Using CRISPick36, we designed five guide RNAs (gRNAs) targeting each gene’s promoter and cloned them into an adeno-associated virus (AAV)-based expression vector. Differentiated adipocytes derived from human white preadipocytes were co-transfected with the gRNAs along with a Staphylococcus aureus endo
Keywords: adipocytes crispra fig organoids ucp1
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